p-stat3 (y705) antibody Search Results


antibodies against p stat3 y705  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc antibodies against p stat3 y705
( A ) Log2(fold-change; Trp53 ko/parental) heat maps for STAT1, <t>STAT3,</t> STAT5, and RelA transcription factor target gene expression from the OVE spheroid RNA-seq dataset . See Supplementary Tables 3–10 for normalized read counts. RT-qPCR validation on cDNA from OVE4 and OVE4- Trp53 ko ( B ) adherent and ( C ) spheroid cells for top hits within the STAT1, STAT3, STAT5, and RelA target gene lists. Representative western blots and densitometric analysis for ( D , E ) p-STAT3, ( F , G ) p-STAT5, and (F, H ) p-RelA in OVE4 and OVE4- Trp53 ko adherent and spheroid cells. ( I ) IFNγ concentrations in acellular peritoneal wash samples from orthotopic injection mouse groups as determined by ELISA. Representative western blots and densitometric analysis for IFNγ-induced p-STAT1 in OVE4 and OVE4- Trp53 ko ( J , K ) adherent and ( L , M ) spheroid cells. Cells were treated with 0, 1, or 10 ng/mL IFNγ for 1 hour. Statistical analyses were performed by unpaired, two-tailed Student’s t -test for RT-qPCR data, and by one-way ANOVA followed by Tukey’s multiple comparisons test for densitometry data ( * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; n = 3). Error bars represent standard error of the mean for RT-qPCR data, and standard deviation for densitometry data.
Antibodies Against P Stat3 Y705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Log2(fold-change; Trp53 ko/parental) heat maps for STAT1, STAT3, STAT5, and RelA transcription factor target gene expression from the OVE spheroid RNA-seq dataset . See Supplementary Tables 3–10 for normalized read counts. RT-qPCR validation on cDNA from OVE4 and OVE4- Trp53 ko ( B ) adherent and ( C ) spheroid cells for top hits within the STAT1, STAT3, STAT5, and RelA target gene lists. Representative western blots and densitometric analysis for ( D , E ) p-STAT3, ( F , G ) p-STAT5, and (F, H ) p-RelA in OVE4 and OVE4- Trp53 ko adherent and spheroid cells. ( I ) IFNγ concentrations in acellular peritoneal wash samples from orthotopic injection mouse groups as determined by ELISA. Representative western blots and densitometric analysis for IFNγ-induced p-STAT1 in OVE4 and OVE4- Trp53 ko ( J , K ) adherent and ( L , M ) spheroid cells. Cells were treated with 0, 1, or 10 ng/mL IFNγ for 1 hour. Statistical analyses were performed by unpaired, two-tailed Student’s t -test for RT-qPCR data, and by one-way ANOVA followed by Tukey’s multiple comparisons test for densitometry data ( * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; n = 3). Error bars represent standard error of the mean for RT-qPCR data, and standard deviation for densitometry data.

Journal: Oncotarget

Article Title: Loss of Trp53 results in a hypoactive T cell phenotype accompanied by reduced pro-inflammatory signaling in a syngeneic orthotopic mouse model of ovarian high-grade serous carcinoma

doi: 10.18632/oncotarget.28768

Figure Lengend Snippet: ( A ) Log2(fold-change; Trp53 ko/parental) heat maps for STAT1, STAT3, STAT5, and RelA transcription factor target gene expression from the OVE spheroid RNA-seq dataset . See Supplementary Tables 3–10 for normalized read counts. RT-qPCR validation on cDNA from OVE4 and OVE4- Trp53 ko ( B ) adherent and ( C ) spheroid cells for top hits within the STAT1, STAT3, STAT5, and RelA target gene lists. Representative western blots and densitometric analysis for ( D , E ) p-STAT3, ( F , G ) p-STAT5, and (F, H ) p-RelA in OVE4 and OVE4- Trp53 ko adherent and spheroid cells. ( I ) IFNγ concentrations in acellular peritoneal wash samples from orthotopic injection mouse groups as determined by ELISA. Representative western blots and densitometric analysis for IFNγ-induced p-STAT1 in OVE4 and OVE4- Trp53 ko ( J , K ) adherent and ( L , M ) spheroid cells. Cells were treated with 0, 1, or 10 ng/mL IFNγ for 1 hour. Statistical analyses were performed by unpaired, two-tailed Student’s t -test for RT-qPCR data, and by one-way ANOVA followed by Tukey’s multiple comparisons test for densitometry data ( * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; n = 3). Error bars represent standard error of the mean for RT-qPCR data, and standard deviation for densitometry data.

Article Snippet: Antibodies against p-STAT3-Y705 (CAT# 9131; 1:1000), STAT3 (CAT# 12640; 1:1000), p-STAT5-Y694 (CAT# 4322; 1:1000), STAT5 (CAT# 9363; 1:1000), p-RelA-S536 (CAT# 3033; 1:1000), RelA (CAT# 8242; 1:1000), p-STAT1-Y701 (CAT# 9167; 1:1000), and STAT1 (CAT# 9172; 1:1000) were purchased from Cell Signaling Technologies.

Techniques: Targeted Gene Expression, RNA Sequencing, Quantitative RT-PCR, Biomarker Discovery, Western Blot, Injection, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Standard Deviation

RT-qPCR validation on cDNA from OVE4- Trp53 ko and Asc24 ( A ) adherent and ( B ) spheroid cells for inflammatory target genes. Representative western blots and densitometric analysis for ( C , D ) p-STAT3, ( E , F ) p-STAT5, and (E, G ) p-RelA in OVE4- Trp53 ko and Asc24 adherent and spheroid cells. Representative western blots and densitometric analysis for IFNγ-induced p-STAT1 in OVE4- Trp53 ko and Asc24 ( H , I ) adherent and ( J , K ) spheroid cells. Cells were treated with 0 or 1 ng/mL IFNγ for 1 hour. Statistical analyses were performed by unpaired, two-tailed Student’s t -test for RT-qPCR data and p-STAT3, p-STAT5, and p-RelA densitometry data. Statistical analyses were performed by one-way ANOVA followed by Tukey’s multiple comparisons test for p-STAT1 densitometry data ( * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; n = 3). Error bars represent standard error of the mean for RT-qPCR data, and standard deviation for densitometry data.

Journal: Oncotarget

Article Title: Loss of Trp53 results in a hypoactive T cell phenotype accompanied by reduced pro-inflammatory signaling in a syngeneic orthotopic mouse model of ovarian high-grade serous carcinoma

doi: 10.18632/oncotarget.28768

Figure Lengend Snippet: RT-qPCR validation on cDNA from OVE4- Trp53 ko and Asc24 ( A ) adherent and ( B ) spheroid cells for inflammatory target genes. Representative western blots and densitometric analysis for ( C , D ) p-STAT3, ( E , F ) p-STAT5, and (E, G ) p-RelA in OVE4- Trp53 ko and Asc24 adherent and spheroid cells. Representative western blots and densitometric analysis for IFNγ-induced p-STAT1 in OVE4- Trp53 ko and Asc24 ( H , I ) adherent and ( J , K ) spheroid cells. Cells were treated with 0 or 1 ng/mL IFNγ for 1 hour. Statistical analyses were performed by unpaired, two-tailed Student’s t -test for RT-qPCR data and p-STAT3, p-STAT5, and p-RelA densitometry data. Statistical analyses were performed by one-way ANOVA followed by Tukey’s multiple comparisons test for p-STAT1 densitometry data ( * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; n = 3). Error bars represent standard error of the mean for RT-qPCR data, and standard deviation for densitometry data.

Article Snippet: Antibodies against p-STAT3-Y705 (CAT# 9131; 1:1000), STAT3 (CAT# 12640; 1:1000), p-STAT5-Y694 (CAT# 4322; 1:1000), STAT5 (CAT# 9363; 1:1000), p-RelA-S536 (CAT# 3033; 1:1000), RelA (CAT# 8242; 1:1000), p-STAT1-Y701 (CAT# 9167; 1:1000), and STAT1 (CAT# 9172; 1:1000) were purchased from Cell Signaling Technologies.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Western Blot, Two Tailed Test, Standard Deviation